| Primer | Species | LP | RP | Tml | Tmr | Length | Score ⇅ | On-target ⇅ | RI genome sensitivity ⇅ |
|---|---|---|---|---|---|---|---|---|---|
| Primer 51 | Bradyrhizobium diazoefficiens | TGCTGCTGTTCACCACGAA | ACATTGAGCGACCGCAGT | 60.15 | 59.66 | 169 | 64 |
100.00%
|
100.00%
|
| Primer 52 | Bradyrhizobium diazoefficiens | TTGCCATGCAGGTGCTGT | GGCGTCTTCGTCCAACTCA | 60.20 | 60.01 | 239 | 64 |
100.00%
|
100.00%
|
| Primer 53 | Bradyrhizobium diazoefficiens | AATGCTGCTCGACGCGAT | GCTCACGCAGGATGTTGGA | 60.20 | 60.38 | 293 | 64 |
100.00%
|
100.00%
|
| Primer 54 | Bradyrhizobium diazoefficiens | TCGTAGCCGTCGGTTATGC | ACGATGCCGAACACCGAA | 59.94 | 59.66 | 151 | 64 |
100.00%
|
100.00%
|
| Primer 55 | Bradyrhizobium diazoefficiens | TGACCGCATTCCCGTAACC | TCCTGCCATTGTCGCGAA | 60.08 | 59.65 | 170 | 64 |
100.00%
|
100.00%
|
| Primer 56 | Bradyrhizobium diazoefficiens | GACTGACCGCATTCCCGTA | TCCTGCCATTGTCGCGAA | 59.49 | 59.65 | 173 | 64 |
100.00%
|
100.00%
|
| Primer 57 | Bradyrhizobium diazoefficiens | TCTTGCTCGATGCGCTGA | CAGAGCAGGCAGAACACCA | 59.43 | 59.93 | 211 | 64 |
100.00%
|
100.00%
|
| Primer 58 | Bradyrhizobium diazoefficiens | TCTTGCTCGATGCGCTGA | AGAGCAGGCAGAACACCAC | 59.43 | 59.93 | 210 | 64 |
100.00%
|
100.00%
|
| Primer 59 | Bradyrhizobium diazoefficiens | TCTTGCTCGATGCGCTGA | ACCAGAGCAGGCAGAACAC | 59.43 | 59.93 | 213 | 64 |
100.00%
|
100.00%
|
| Primer 60 | Bradyrhizobium diazoefficiens | TCTTGCTCGATGCGCTGA | CCGAACCACCACAGGAACA | 59.43 | 59.85 | 298 | 64 |
100.00%
|
100.00%
|